Bo-rasaense ba thehile sethala sa 3D bioprinting se kopanyang ts'ebetso botho lisele tsa neural. Lisele tsa progenitor ka har'a lisele tse hatisitsoeng li hola ho theha li-circuits tsa neural mme li etsa likhokahano tse sebetsang le li-neurone tse ling, kahoo li etsisa tlhaho. boko lisele. Ena ke tsoelo-pele e kholo ho boenjiniere ba lisele tsa methapo ea kutlo le ho theknoloji ea 3D bioprinting. Li-tissue tse joalo tsa bioprinted neural li ka sebelisoa ho etsa mohlala botho mafu (a kang Alzheimer's, Parkinson's joalo-joalo) a bakoang ke ho senyeha ha methapo ea kutlo. Patlisiso efe kapa efe ea lefu la boko e hloka ho utloisisa hore na botho marang-rang a neural a sebetsa.
3D bioprinting ke ts'ebetso ea tlatsetso moo biomaterial e loketseng ea tlhaho kapa ea maiketsetso (bioink) e kopantsoeng le lisele tse phelang 'me e hatisitsoe, lera-ka-lera, ka har'a lisele tsa tlhaho tse kang tse tharo-dimensional. Lisele li hola ka har'a bioink mme libopeho li hola ho etsisa lisele kapa setho sa tlhaho. Theknoloji ena e fumane lisebelisoa ho regenerative meriana bakeng sa bioprinting ea lisele, lisele le litho tsa 'mele le lipatlisisong e le mohlala oa ho ithuta botho mele oa a vitro, haholo-holo botho tsamaiso ea methapo.
Thuto ea botho tsamaiso ea methapo e tobana le mefokolo ka lebaka la ho se fumanehe ha lisampole tsa mantlha. Mehlala ea liphoofolo e na le thuso empa e na le ho se tšoane ho itseng ha mefuta ea liphoofolo ka hona ho bohlokoa ho a vitro mehlala ea botho tsamaiso ea methapo ea kutlo ho batlisisa kamoo ho botho marang-rang a neural a sebetsa ho fumana liphekolo tsa mafu a bakoang ke ho senyeha ha marang-rang a methapo ea kutlo.
Human li-neural tissues li 'nile tsa hatisoa ka 3D nakong e fetileng ho sebelisa lisele tsa stem empa tsena li ne li se na neural network formation. Lithishu tse hatisitsoeng li ne li sa bonts'a hore li entse likhokahano lipakeng tsa lisele ka mabaka a 'maloa. Mefokolo ena e hlōtsoe hona joale.
Phuputsong ea haufinyane, bafuputsi e khethile fibrin hydrogel (e nang le fibrinogen le thrombin) e le bioink ea mantlha mme e rerile ho hatisa sebopeho se nang le lera moo lisele tsa progenitor li ka holang le ho etsa li-synapse ka har'a le ka har'a lihlopha, empa li fetotse tsela eo marako a pakoang ka eona nakong ea khatiso. Ho e-na le mokhoa oa setso oa ho bokella lihlopha ka holimo, ba ile ba khetha ho hatisa lihlopha tse haufi le tse ling tse tšekaletseng. Kamoo ho bonahalang kateng, sena se ile sa etsa phapang. Sethala sa bona sa 3D bioprinting se fumanoe se sebetsa hantle botho lisele tsa methapo ea kutlo. Ntlafatso ea li-platform tse ling tse teng, the botho methapo ea kutlo e hatisitsoeng ke sethala sena e thehile marang-rang a methapo le likhokahano tse sebetsang le li-neuron tse ling le lisele tsa glial kahare le lipakeng tsa mekhahlelo. Ena ke nyeoe ea pele e joalo 'me ke mohato oa bohlokoa ho ea pele boenjiniere ba methapo ea kutlo. Tšebeliso ea laboratori ea methapo ea kutlo e etsisang boko ha e sebetsa e utloahala e thabisa. Ka sebele tsoelo-pele ena e tla thusa bafuputsi tabeng ea ho etsa mohlala botho mafu a boko a bakoang ke ho senyeha ha marang-rang a methapo ea kutlo ho utloisisa hamolemo mokhoa oa ho fumana phekolo e ka khonehang.
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References:
- Cadena M., et al a 2020. 3D Bioprinting ea Neural Tissues. Lisebelisoa tse Tsoetseng Pele tsa Bophelo bo Botle Volume 10, Khatiso ea 15 2001600. DOI: https://doi.org/10.1002/adhm.202001600
- Yan Y., et al a 2024. 3D bioprinting ea botho li-neural tissues tse nang le khokahanyo e sebetsang. Cell Stem Cell Technology| Buka ea 31, Khatiso ea 2, P260-274.E7, Hlakola 01, 2024. DOI: https://doi.org/10.1016/j.stem.2023.12.009
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